Composite

Part:BBa_K3453115:Design

Designed by: Maeva Cherriere   Group: iGEM20_Evry_Paris-Saclay   (2020-10-04)


DmMatK Toehold Switch 2.2 with sfGFP-LVAtag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 35
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 35
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 35
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 35
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 35
    Illegal AgeI site found at 141
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter (BBa_K2150031) + DmMatK Toehold Switch 2.2 (BBa_K3453015) + sfGFP-LVAtag coding sequence (BBa_K2675006) + the strong SBa_000587 Terminator (BBa_K3453000)


Source

DNA synthesis & Assembly

References